Monooxygenase and glucuronyltransferase activities in short term cultures of isolated rat hepatocytes
✍ Scribed by L.R. Schwarz; R. Götz; Th. Wolff; F.J. Wiebel
- Book ID
- 115909471
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 393 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0014-5793
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The determination of the mixed function flavin-containing monooxygenase activity in rat liver and in hepatocytes and their cultures by spectrophotometric measurement of the oxygenation of methimazole is complicated by an inhibition caused by some of the reagents used during this method. Optimal cond
The apparent kinetics of uptake of various model substrates were examined for hepatocytes in suspension and primary culture up to 72 h. The ability of hepatocytes to take up taurocholate and ouabain was decreased in culture. Vmax for uptake of both substrates diminished rapidly with increasing time