Monocyte chemoattractant protein-1 enhances expression of intercellular adhesion molecule-1 following ischemia-reperfusion of the liver in rats

✍ Scribed by Yasuo Yamaguchi; Fujio Matsumura; Motohiro Takeya; Osamu Ichiguchi; Jun-Ichi Kuratsu; Tadashi Horiuchi; Eiji Akizuki; Teishi Matsuda; Kazutoshi Okabe; Hajime Ohshiro; Jian Liang; Katsutaka Mori; Shinwa Yamada; Kiyoshi Takahashi; Michio Ogawa

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 172 KB
• Volume: 27
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.510270314

✦ Synopsis

Intercellular adhesion molecule-1 (ICAM-1) is important in neutrophil-dependent injury. We investigated the effects of monocyte chemoattractant protein-1 (MCP-1) produced by Kupffer cells on ICAM-1 expression after ischemia-reperfusion in rat liver by occluding the portal vein for 30 minutes. Serum concentrations of MCP-1 increased persistently. By Northern analysis, MCP-1 mRNA increased early and persisted. Kupffer cells harvested 6 hours after reperfusion also expressed this transcript. The transcript and protein also were produced by Kupffer cells from naive controls in response to reactive oxygen species. ICAM-1 mRNA transcripts increased, peaked 3 hours after reperfusion, and decreased gradually thereafter. The level of ICAM-1 mRNA transcripts in the WK-5 rat endothelial cell line were markedly enhanced by MCP-1. These results suggest that MCP-1 released by Kupffer cells early after ischemia-reperfusion modulates neutrophil-dependent tissue injury via ICAM-1.