Monoclonal autoantibody from a (new zealand black x new zealand white)F1 mouse and some human scleroderma sera target an MTr 34,000 nucleolar protein of the U3 RNP particle
✍ Scribed by G. Reimer; K. M. Pollard; C. A. Penning; R. L. Ochs; M. A. Lischwe; H. Busch; E. M. Tan
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 897 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
A monoclonal IgG2a antinucleolar autoantibody (72B9) was obtained by fusion of spleen cells from a (New Zealand black X New Zealand white)F1 mouse with myeloma cells (P3x63Ag8.653). Antibody 72B9 recognized a highly conserved nucleolar antigen present in both animal and plant cells. The staining pattern produced by antibody 72B9 in different cell substrates was identical with those obtained by scleroderma antibodies reactive with a basic (PI 8.5) nucleolar protein of M, 34,000, which is associated with the U3 RNP particle. Western blotting further confirmed its reactivity with this scleroderma-related U3 RNP protein.
Monoclonal antibodies against nuclear antigens, such as DNA, histones, Sm, U1 RNP, and SS-B (La), which are targets of spontaneously occurring autoantibodies in systemic rheumatic diseases, have Publication 4462-BCR from the W.