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Monoclonal antibody αIR-3 inhibits non-small cell lung cancer growth in vitro and in vivo

✍ Scribed by Farah Zia; Steve Jacobs; Frederick Kull Jr.; Frank Cuttitta; James L. Mulshine; Dr. Terry W. Moody

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
601 KB
Volume
63
Category
Article
ISSN
0730-2312

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✦ Synopsis

The ability of monoclonal antibody (mAb) alR-3 to interact with non-small cell lung cancer (NSCLC) cells was investigated. MAb alR-3 inhibited specific binding of 1251-IGF-I and 1251-alR-3 to a panel of 8 NSCLC cell lines with high affinity (IC50 = 200 and 50 ng/ml, respectively). 1251-alR-3 bound with high affinity (Kd = 40 ng/ml) to a single class of sites (Bmax = 8,00O/cell) using NCLH838 cells. 1251-alR-3 was internalized when exposed to N O H 8 3 8 or H I 2 9 9 cells at 37°C but not 4°C. alR-3 irnmunoprecipitated major 9 0 and 130 kD proteins. IGF-I stimulated and alR-3 inhibited the clonal growth of NCI-HI299 cells. aIR-3 slowed the growth of NCI-HI57 and H838 xenografts in nude mice. In a biodistribution study 1251-alR-3 was preferentially localized to the tumor as opposed to other organs. These data suggest that IGF-I may be a regulatory agent in NSCLC.

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