Monoclonal antibody technology for cyclosporine monitoring

✍ Scribed by Valerie F.J. Quesniaux

• Book ID: 103039202
• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 441 KB
• Volume: 24
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(91)90147-7

✦ Synopsis

Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with highperformance liquid chromatographic measurements of CsA. Monoclonal antibodies (mAbs) have the potential to discriminate between closely related molecules. MAbs with high affinity for CsA have been prepared and their fine-specificity characterized by cross-reactivity studies using a large series of CsA-derivatives. According to the known sites of metabolism on the CsA molecule and to its threedimensional structure, it was possible to predict which mAb would be suitable for recognizing native Cs specifically.