Monoclonal antibodies to human malignant mesothelioma
โ Scribed by Timothy M. Anderson; E. Carmack Holmes; Calvin J. Kosaka; Lorna Cheng; Romaine E. Saxton
- Book ID
- 105003789
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 732 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0271-9142
No coin nor oath required. For personal study only.
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Monoclonal antibodies (MAbs) were generated by immunizing mice with the mesothelioma cell line SPC111 and selected by indirect immunofluorescence on viable cells. Indirect immunofluorescence staining and radioimmunoassays demonstrated selective binding of the antibodies ME1 and ME2 with the surface
The technology for the production of murine monoclonal antibodies has been refined enormously since its introduction in 1975. However, the technology for generating human monoclonal antibodies has only recently come into its own. In this review, three currently available approaches to the production