✦ LIBER ✦

Monoclonal antibodies to a brain dopamine binding protein: Production, specificity, and immunohistochemistry

✍ Scribed by Dr. L. L. Hsu; K. Moroi; B. D. Lakchaura; T. Chan; C.-T. Lin; J.-Y. Wu

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 673 KB
Volume: 17
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490170413

No coin nor oath required. For personal study only.

✦ Synopsis

A dopamine binding protein (DABP) has been purified from the rat brain synaptic membrane to homogeneity by affinity chromatography and gel electrophoresis. The monoclonal antibodies against the DABP were produced by the mouse-mouse hybridoma technique and characterized for their specificity to dopamine receptors by displacement of dopamine receptor binding. These monoclonal antibodies have been used to localize DABP in rat brain by immunohistochemistry. A specific linear structure of reaction product was seen in both caudate nucleus and cerebral cortex. This finding suggests that the DABP is present in the cerebral cortex and caudate nucleus as a membranous component of the neurons or their processes.

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