Monoclonal antibodies developed againstBotryllus blood cell antigens bind to cells of distinct lineages during embryonic development

✍ Scribed by Schlumpberger, J. M. ;Weissman, I. L. ;Scofield, V. L.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 826 KB
• Volume: 229
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402290205

✦ Synopsis

Monoclonal antibodies that bind to adult Botryllus blood cell surface antigens were used to detect and localize identical or cross-reacting antigenic determinants on Botryllus embryonic cells. These anti-Botr-yllus monoclonal reagents could be divided into three categories: 1) those that bound to most embryonic cell types, including all very early embryonic cells; 2) those that bound oiily to embryonic blood cells and a few other embryonic cell types; and 3) those that did not bind detectably to any embryonic cells.

In 1905 Conklin published "Organization and Cell-Lineage of the Ascidian Egg" (Conklin, '05a), a foundation work in embryology. Because of these early studies by Conklin and others (