Monoclonal antibodies against human recombinant tumor necrosis factor: prevention of endotoxic shock
β Scribed by Yoshinori Shimamoto; Rita L. Chen; Arthur Bollon; Alan Chang; Amanullah Khan
- Book ID
- 107906141
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 523 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0165-2478
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Cation-exchange liquid chromatography (CIEX) and capillary isoelectric focusing (cIEF) methods have been developed for the routine analysis of a recombinant, human anti-tumor necrosis factor monoclonal antibody D2E7. Both of these methods can separate heavy-chain C-terminal variants of this antibody
By in situ FTIR ATR measurements, the antibody (AB) recognition of human tumor necrosis factor-a (TNFa) immobilized on the Ge surface of a multiple internal reflection element (MIRE) was investigated. The experiments were performed in aqueous environment in a flow-through cell. After immobilization