Monoclonal Antibody Protocols
β William C. Davis
π Library
π
1995
π Humana Press
π English
β¦ LIBER β¦
π
Monoclonal Antibodies
β Scribed by J. H. Peters, D. Baron (auth.), Professor Dr. med. Johann Hinrich Peters, Dr. rer. nat. Horst Baumgarten (eds.)
- Publisher
- Springer-Verlag Berlin Heidelberg
- Year
- 1992
- Tongue
- English
- Leaves
- 503
- Series
- Springer Laboratory
- Edition
- 1
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proceΒ dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, sucΒ ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.
β¦ Table of Contents
Front Matter....Pages I-XVII
Introduction....Pages 1-17
Preconditions for Hybridoma Technology....Pages 18-38
Immunization....Pages 39-70
Taking Blood and Isolating Cells....Pages 71-87
Cell Culture....Pages 88-136
Production of Hybridomas....Pages 137-222
Mass Production of Monoclonal Antibodies....Pages 223-257
Purifying Monoclonal Antibodies and Producing Antibody Fragments....Pages 258-284
Coupling Monoclonal Antibodies....Pages 285-315
Demonstration of Monoclonal Antibodies....Pages 316-461
Safety Precautions at Work....Pages 463-465
Appendix....Pages 466-474
Back Matter....Pages 475-488
β¦ Subjects
Immunology; Biochemistry, general; Cell Biology; Anatomy
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