Mono and two-dimensional 500-MHz characterization of synthetic bombesin and related peptides in DMSO and DMSO–water,
✍ Scribed by Carlo Di Bello; Luigia Gozzini; Mauro Tonellato; Maria Grazia Corradini; Gabriella D'Auria; Livio Paolillo; Enrico Trivellone
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1989
- Tongue
- English
- Weight
- 784 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
The proton nmr characterization of bombesin (BBS) and of two peptide fragments corresponding to the (1-6) and (6-14) sequences has been carried out at 500 MHz in dimethyl sulfoxide (DMSO-d,) using two-dimensional (2D) homo and 'H-13C hetermorrelated techniques. All resonances in the nmr spectra have been assigned and several coupling constants have been measured. The backbone JmCH--NH coupling constants are quite similar and around 7.8-8.2 Hz, pointing to an unfolded structure in DMSO-d,. The possibility of secondary structures in highly viscous mixtures of DMSo-d,-water was investigated. The existence of sequentid nuclear Overhauser enhancement (NOE) effects in the C-terminal nonapeptide section may indicate a preferential Site for secondary structuring. *A preliminary report of this work was presented at the Japan Symposium on Peptide +For standard abbreviations for amino acids and peptides refer to IUF'AC-IUB (1984) Ew. J.