Monitoring the non-specific interactions of catechin through diffusion measurements based on pulsed-field gradients
✍ Scribed by C. Monteiro; C. Maechling; C. Hervé du Penhoat
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 121 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.1108
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✦ Synopsis
Abstract
The self‐association of aqueous catechin as a function of concentration was monitored through variations in ^1^H chemical shifts, proton T~1~ and T~2~ data and translational diffusion coefficients obtained with the pulsed‐field gradient spin‐echo method. The latter approach is very efficient and it is not restricted to aromatic compounds. Equilibrium constants were estimated for various models of self‐association and the apparent enthalpy of dissociation was measured with isothermal titration calorimetry. Comparison of the latter parameter with thermodynamic data reported for various types of non‐specific interactions suggests that such phenomena could be studied using this approach. Copyright © 2002 John Wiley & Sons, Ltd.