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Monitoring the Cellular Surface Display of Recombinant Proteins by Cysteine Labeling and Flow Cytometry

โœ Scribed by Joachim Jose; Steffen Handel


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
200 KB
Volume
4
Category
Article
ISSN
1439-4227

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โœ Claudia Holzer; Peter Maier ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 656 KB

Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto