Monitoring survival and gene transfer in soil microcosms of recombinantEscherichia colidesigned to represent an industrial production strain
✍ Scribed by Rolf B. Henschke; Ellen J. Henschke; Friedrich R. J. Schmidt
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 832 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
✦ Synopsis
A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (nonamended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.