Monitoring Pyridoxine by Capillary Zone Electrophoresis with Electrochemical Detection

✍ Scribed by Wenrui Jin; Jie Zhang

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 73 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/(sici)1521-4109(20000401)12:6<465::aid-elan465>3.0.co;2-r

✦ Synopsis

Capillary zone electrophoresis was employed for the determination of pyridoxine using end-column amperometric detection with a carbon ®ber microelectrode at a constant potential of 0.72 V (vs. SCE). The optimum conditions of separation and detection are 1.0610 72 mol/L NH 4 Cl±1.0610 72 mol/L NH 3 for the buffer solution, 10 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 1.2610 76 mol/L or 3.5 fmol (S/N 2). The relative standard deviation is 1.3 % for the migration time and 3.4 % for the electrophoretic peak current. The method was applied to determining pyridoxine in human urine.