Monitoring of spectroscopic changes of a single trapped fission yeast cell by using a Raman tweezers set-up

✍ Scribed by G. Başar; S. Kın

• Publisher: Elsevier Science
• Year: 2008
• Tongue: English
• Weight: 423 KB
• Volume: 281
• Category: Article
• ISSN: 0030-4018
• DOI: 10.1016/j.optcom.2008.06.074

✦ Synopsis

We demonstrate an improvement of the sensitivity of a Raman tweezers set-up, which combines optical tweezers with Raman spectroscopy. The system was tested by taking the Raman spectrum of a 4.6 lm diameter polystyrene sphere trapped in an aqueous solution. The improvement of sensitivity of the set-up was achieved by adjusting the trap depth for maximum signal to noise ratio (SNR). The maximum SNR was obtained by investigating the Raman peak of a trapped polystyrene sphere at 1001 cm À1 according to trap depth. With this system, a single trapped living Schizosaccharomyces Pombe yeast cell was sensitively monitored by taking the kinetic Raman spectra for more than 2 h. The relative intensity decrease in amide I and amide III bands, frequency increase in amide I band together with alterations in tyrosine marker band around 850 cm À1 was observed, which indicates alterations in the hydration state of protein by time progressing.