Monitoring of solid phase peptide synthesis by FT-IR spectroscopy
β Scribed by Bernd Henkel; Ernst Bayer
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 122 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1075-2617
No coin nor oath required. For personal study only.
β¦ Synopsis
Aggregation phenomena of growing peptides on the resin have seldom been investigated. We report here how conformations are determined by FT-IR spectroscopy. Therefore the sequence 80 -99 of HIV 1-protease was synthesized. After every coupling a resin sample was taken out of the reaction column and a FT-IR spectrum recorded. The results were compared with the UV monitoring obtained from another synthesis of the same peptide.
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