Monitoring of single nicks in duplex DNA by gel electrophoretic mobility-shift assay
β Scribed by Heiko Kuhn; Ekaterina Protozanova; Vadim V. Demidov
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 134 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0173-0835
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## Abstract We have developed a separation technique for DNAβprotein complex based on electrophoretic mobility shift assay (EMSA) by microchip electrophoresis, which we call microchip electrophoretic mobility shift assay (ΞΌEMSA). To evaluate the ΞΌEMSA, we employed recombinant human nuclear factorβΞΊ
The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph