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Monitoring of renal allograft recipients by quantisation of human cytomegalovirus genomes in peripheral blood leukocytes

✍ Scribed by Dr. J. E. Kühn; T. Wendland; P. Schäfer; K. Möhring; U. Wieland; M. Elgas; H. J. Eggers

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
791 KB
Volume
44
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The ratio of human cytomegalovirus (HCMV) genomes per cellular genomes in serial peripheral blood leukocyte (PEL) extracts of renal allograft recipients was quantitated by competitive nested polymerase chain reaction (PCR). Patients were also monitored for the development of acute HCMV infection by detection of HCMV pp65 antigenemia, HCMV IgM antibodies, and viruria. Compared to qualitative nested HCMV PCR, the frequency of positive PCR results in renal allograft recipients without further evidence of acute HCMV infection was significantly reduced by quantitative HCMV PCR. HCMV DMA levels ≥1,000 copies HCMV/10^6^ copies β‐globin were found to be highly indicative for the development of a clinically symptomatic HCMV infection following renal allograft transplantation. In patients treated with ganciclovir, quantitation of HCMV target sequences allowed the assessment of the efficacy of antiviral therapy. © 1994 Wiley‐Liss, Inc.

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