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Monitoring of membrane potential changes inSaccharomyces cerevisiaeby diS-C3(3) fluorescence

✍ Scribed by B. Denksteinová; D. Gášková; P. Heřman; J. Večeř; J. Malínský; J. Plášek; K. Sigler


Book ID
105621206
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
326 KB
Volume
42
Category
Article
ISSN
0015-5632

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Membrane-potential-dependent accumulation of diS-C 3 (3) in intact yeast cells in suspension is accompanied by a red shift of the maximum of its fluorescence emission spectrum, max , caused by a readily reversible probe binding to cell constituents. Membrane depolarization by external KCl (with or w