Monitoring of enzymatic hydrolysis of ivory nut mannan using on-line microdialysis sampling and anion-exchange chromatography with integrated pulsed electrochemical detection

✍ Scribed by Nelson Torto; Torbjörn Buttler; Lo Gorton; György Marko-Varga; Henrik Stålbrand; Folke Tjerneld

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 892 KB
• Volume: 313
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00071-7

✦ Synopsis

A new system for on-line monitoring of a small-scale bioprocess is described, based on microdialysis sampling and anion-exchange chromatography with integrated pulsed electrochemical detection. The microdialysis probe equipped with a 16-mm polysulfone membrane was used as an in situ sampling unit, giving a clean dialysate with no further need for sample clean-up. The system was used to continuously monitor the hydrolysis of 0.25% ivory nut mannan using endo-1,4-P-mannanase (mannan endo-1,4+mannosidase; EC 3.2.1.78) from Aspergillus niger. The oligosaccharides produced in the hydrolysis, 1,4-P-D-mannobiose, mannotriose, mannotetraose, and mannopentaose, were separated isocratically and detected electrochemically.

On-line monitoring of the hydrolysis of mannan using the endo-mannanase from A. niger, in a 5-ml reaction vessel, was achieved using 50 mM NaOH as mobile phase for 32 h without sampling or detection problems.