Monitoring of adenovirus from conjunctival scrapings in Japan during 2005–2006

✍ Scribed by Kiyohiko Matsui; Hideaki Shimizu; Atsushi Yoshida; Emi Nagaoka; Osamu Nishio; Kenji Okuda

• Book ID: 102378086
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 145 KB
• Volume: 80
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.21175

✦ Synopsis

Abstract

A total of 189 conjunctival scrapings were collected from patients in Tokyo, Japan by monitoring adenovirus infection in community‐based clinics during 2005 and 2006. Of the 189 samples, 155 (82%) had adenoviruses detected by polymerase chain reaction (PCR). The serotypes were determined by PCR‐restriction fragment length polymorphism (PCR‐RFLP) analysis, using a combination of endonucleases, such as __Hha__I, __Alu__I, and __Hae__III, and neutralization tests (NTs). PCR‐RFLP identified five serotypes: serotype 3: 16.8%, serotype 4: 9.7%, serotype 8: 34.8%, serotype 11: 23.2%, and serotype 37: 15.5%. Adenovirus 8 was the most common serotype identified. A subset consisting of 25 isolates identified as adenovirus 8 from this study plus 25 isolates from Kawasaki were analyzed using PCR sequencing of the hexon gene. Compared with prototype adenovirus serotype 8 and serotype 9 derived in Tokyo and Kawasaki, these isolates shared 61.7–62.8% and 80.5–82.7% amino acid homology, respectively, suggesting that a variant adenovirus serotype 8 was involved in this outbreak, and is different from the prototype adenovirus 8 virus. This variant had not been detected in Japan prior to 1996 and appears to be the most common adenovirus type 8 involved in cases of epidemic keratoconjunctivitis in Japan at present. J. Med. Virol. 80:997–1003, 2008. © 2008 Wiley‐Liss, Inc.