✦ LIBER ✦

Monitoring human exposure to carcinogens by DNA adduct measurement

✍ Scribed by Regina M. Santella

Book ID: 104627586
Publisher: Springer
Year: 1988
Tongue: English
Weight: 346 KB
Volume: 4
Category: Article
ISSN: 0742-2091
DOI: 10.1007/bf00117779

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✦ Synopsis

Sensitive immunologic techniques are now available for the detection and quantitation of carcinogen-DNA adducts. We have developed a number of specific monoclonal antibodies which recognize DNA modified by particular carcinogens, including benzo[a]pyrene, aminopyrene, 8-methoxypsoralen plus UVA light and vinyl chloride. These antibodies can be used in competitive enzyme-linked immunosorbent assays to quantitate adducts in DNA isolated from biological samples. Samples from treated animals as well as from humans with occupational or environmental exposure to carcinogens have been studied. In addition, antibodies can be used in indirect immunohistochemical studies to localize adduct formation in various tissues or cell types.

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