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Monitoring folding transitions of synthetic, branched-chain polypeptides by capillary zone electrophoresis

✍ Scribed by Barbara Verzola; Massimiliano Perduca; Gábor Mezô; Ferenc Hudecz; Pier Giorgio Righetti


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
212 KB
Volume
24
Category
Article
ISSN
0173-0835

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Monitoring Equilibria and Kinetics of Pr
✍ Barbara Verzola; Fabrizio Chiti; Giampaolo Manao; Pier Giorgio Righetti 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 92 KB

A method is described here for studying conformational transitions of proteins due to denaturing agents: capillary zone electrophoresis (CZE) in acidic, isoelectric buffers. The sample is run in 50 mM isoelectric glutamic acid (pH ‫؍‬ pI ‫؍‬ 3.2) added with 1 mM oligoamine (tetraethylene pentamine)