Monitoring and quantification on solid support of a by-product formation during peptide synthesis by Tof-SIMS

✍ Scribed by C. Enjalbal; D. Maux; G. Subra; J. Martinez; R. Combarieu; J.-L. Aubagnac

• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 228 KB
• Volume: 40
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(99)01158-2

✦ Synopsis

To evaluate the amount of pyroglutamic acid formation during the synthesis of glutamic acid containing peptides, resin beads were substituted by known amounts of Fmoc-Glu((YBu)-Phe-Ala-NH2 and the corresponding sideproduct Pyr-Phe-Ala-NH2 and directly analyzed by time of flight-secondary ion mass spectrometry. Detection and quantification of pyroglutamic acid was achieved without release of the peptide chains in solution.