𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Monitoring and Optimizing Detergent Concentration For Membrane Protein Crystallization While Following Protein Homogeneity

✍ Scribed by Miercke, Larry J.W.; Robbins, Rebecca A.; Ho, Mimi; Sandstrom, Andrew; Bond, Rachel K.; Stroud, Robert M.

Book ID
119206349
Publisher
Biophysical Society
Year
2010
Tongue
English
Weight
35 KB
Volume
98
Category
Article
ISSN
0006-3495

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Validation of the detergent micelle clas
Validation of the detergent micelle classification for membrane protein crystals and explanation of the Matthews Graph for soluble proteins
✍ Georg E. Schulz πŸ“‚ Article πŸ“… 2011 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 295 KB

## Abstract Protein crystals are of wide‐spread interest because many of them allow structure analyses at atomic resolution. For soluble proteins, the packing density of such crystals is distributed according to the Matthews Graph. For integral membrane proteins, the respective graph is similar but

A single-step method to concentrate and
A single-step method to concentrate and desalt proteins which is also useful for determination of detergent binding to membrane proteins
✍ Emilio Rivas; Nicole Pasdeloup; Marc le Maire πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 568 KB

The conditions for obtaining highly concentrated salt-free protein solutions have been examined. It was found that a convenient and rapid method consists of combining ion-exchange and gel-filtration chromatography in one column. We observed that a volume ratio of Sephadex G-25 to ion exchanger of 2.