Monensin-sensitive cellular events modulate neurite extension on laminin: An example of higher-order regulation of cell motility
✍ Scribed by Smalheiser, Neil R.
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 770 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0886-1544
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✦ Synopsis
NG108-15 cells extend "rapid-onset'' neurites vigorously within the first hour after plating in minimal serum-free medium on Petri dishes coated with polylysine and laminin (1 ng/mm2). We recently reported that the initial rates of neurite formation and cell translocation are further accelerated in this system when nonspecific substratum attachment sites are partially blocked by polyglutamate, bovine serum albumin, or polyethylene glycol polymers [Smalheiser, N. R. (1991): Dev. Brain Res. 62:81-891. When cells were plated in the presence of the monovalent cation ionophore monensin (1-5 pM) or hypertonic sucrose (50-100 &), the initial rate of outgrowth on laminidpolylysine-treated Petri dishes was not affected, yet the acceleration produced by polyglutamate was strongly inhibited.
These data indicate that monensin-sensitive intracellular events can regulate neurite extension on laminin indirectly, through modulating the effects exerted on cells by nonspecific substratum sites. Although the critical events affected by monensin remain to be identified, movements of laminin receptors (their clustering, internalization, and recycling) are likely targets for further study.