Molecularly imprinted polymeric membranes having EFF derivatives as a chiral recognition site

✍ Scribed by Masakazu Yoshikawa; Takashi Fujisawa; Jun-ichiro Izumi

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 102 KB
• Volume: 200
• Category: Article
• ISSN: 1022-1352
• DOI: 10.1002/(sici)1521-3935(19990601)200:6<1458::aid-macp1458>3.0.co;2-d

✦ Synopsis

Molecularly imprinted polymeric membranes with the tripeptide residue H-Glu(OBzl)-Phe-Phe-CH 2 -(EFF) were prepared during the membrane preparation (casting) process in the presence of a print molecule. The Ac-L-Trp imprinted polymeric membranes thus obtained showed adsorption selectivity toward Ac-L-Trp. From adsorption isotherms of Ac-Trp isomers it is seen that the chiral recognition site formed in the presence of the print molecule in the membrane preparation process exclusively recognizes the isomer Ac-L-Trp which was the print molecule, while the opposite isomer, Ac-D-Trp, is rigorously rejected. The affinity constant between Ac-L-Trp and the chiral recognition site was determined to be 9.6610 3 mol -1 N dm 3 . Enantioselective permeation can be attained by applying electrodialysis as a driving force. An optimum separation factor toward the L-isomer of 6.8, which corresponds to the adsorption selectivity, can be reached.