Molecular weight characterization of PRG4 proteins using multi-angle laser light scattering (MALLS)
β Scribed by Steele, B.L.; Alvarez-Veronesi, M.C.; Schmidt, T.A.
- Book ID
- 123015745
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 449 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1063-4584
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## Abstract Two methods for the characterization of protein molecular weights from their diffusion coefficients are discussed. These measurements can be made quickly and reliably at low concentrations using quasielastic lightβscattering techniques. First, an empirical calibration of the diffusion c
Size exclusion chromatography with an inline multi-angle light scattering detector (SEC-MALS) was assessed as a means to characterize and monitor the formation of soluble, high-molecular-weight (HMW) protein aggregates so as to better quantify and model nonnative aggregation kinetics. Assay configur