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Molecular topology of the Photosystem II chlorophyllabinding protein, CP 43: Topology of a thylakoid membrane protein

โœ Scribed by Richard T. Sayre; Elizabeth A. Wrobel-Boerner


Publisher
Springer
Year
1994
Tongue
English
Weight
879 KB
Volume
40
Category
Article
ISSN
0166-8595

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โœฆ Synopsis


We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the amino and carboxyl termini located on the stromal membrane surface, is predicted. This structure is similar to that predicted for CP 47, a PS II chlorophyll a binding protein (Bricker T (1990) Photosynth Res 24: 1-13). The model is discussed in reference to the possible location of chlorophyll binding sites.


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