Molecular tools for a molecular medicine: analyzing genes, transcripts and proteins using padlock and proximity probes

✍ Scribed by Ulf Landegren; Edith Schallmeiner; Mats Nilsson; Simon Fredriksson; Johan Banér; Mats Gullberg; Jonas Jarvius; Sigrun Gustafsdottir; Fredrik Dahl; Ola Söderberg; Olle Ericsson; Johan Stenberg

• Book ID: 102373364
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 103 KB
• Volume: 17
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/jmr.664

✦ Synopsis

Abstract

Procedures and reagents are needed to specifically detect all the macromolecules that are being identified in the course of genome projects. We discuss how this challenge may be met using a set of ligation‐based reagents termed padlock probes and proximity ligation probes. These probes include elements with affinity for specific nucleic acid and protein molecules, respectively, along with unique identifier DNA sequence elements that encode the identity of the recognized target molecules. The information content of DNA strands that form in the detection reactions are recorded after amplification, allowing the recognized target molecules to be identified. The procedures permit highly specific solution‐phase or localized analyses of large sets of target molecules as required in future molecular analyses. Copyright © 2004 John Wiley & Sons, Ltd.