## Abstract The value of molecular techniques for virology is not in dispute; the issue debated here is whether or not to abandon virus isolation altogether. Modern clinical virology relies on rapid virus detection for timely infection control and antiviral therapy. The role of virus isolation, ine
Molecular techniques should now replace cell culture in diagnostic virology laboratories
β Scribed by Bill Carman
- Book ID
- 104590406
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 47 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1052-9276
- DOI
- 10.1002/rmv.334
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
Cell cultures have been important historically for the development of virology. However, I will argue that they are no longer essential for a diagnostic laboratory and should be replaced with nucleic acid detection methods. This change of technology should be costβneutral once the full costs to the laboratory of cell cultures, including staff time, are calculated. Molecular methods are more sensitive than cell cultures and provide answers much more rapidly, especially for negative samples. Since negative results are frequently as important to our clinical colleagues as positive results, we should change now to routine diagnostic services based upon nucleic acid detection. Copyright Β© 2001 John Wiley & Sons, Ltd.
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