Molecular subtyping of human T-lymphotropic virus type I (HTLV-I) by a nested polymerase chain reaction-restriction fragment length polymorphism analysis of the envelope gene: Two distinct lineages of HTLV-I in Taiwan
✍ Scribed by Yang, Ya-Chien; Hsu, Tsuey-Ying; Liu, Mei-Ying; Lin, Ming-Tseh; Chen, Jen-Yang; Yang, Czau-Siung
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 161 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
KEY WORDS: HTLV-I subtypes; env gene; sub-The major type of human T-lymphotropic virus type-specific nucleotides type I (HTLV-I), generally referred to as the cosmopolitan type, has been grouped into three subtypes (A, B, and C) by phylogenetic analysis of the long terminal repeat sequences of the viral ge-INTRODUCTION nome. Twelve subtype-specific nucleotide varia-Human T-lymphotropic virus type I (HTLV-I) is the tions have been deduced by comparison between etiological agent of adult T-cell leukemia/lymphoma the envelope (env) sequences of 16 HTLV-I strains (ATL) [Poiesz et al., 1980;Yoshida et al., 1982] and is with defined subtypes and 9 Taiwanese HTLV-I closely associated with a neurological disorder named strains. To gain further insights into the molecular HTLV-I-associated myelopathy/tropic spastic parapareepidemiology of HTLV-I and the origin of this virus sis (HAM/TSP) [Gessain et al., 1985; Osame et al., in Taiwan, a rapid method of identification for the 1986]. The virus has also been implicated in other cosmopolitan subtypes was developed by using chronic inflammatory disorders, including some cases a nested polymerase chain reaction (PCR) and of polyarthritis [Nishioka et al., 1989], polymyositis [Evsubsequent restriction fragment length polymorans et al., 1989], Sjo ¨gren's syndrome [Green et al., 1989], phism (RFLP) studies using the two subtype Binfectious dermatitis [LaGrenade et al., 1990], and uvespecific and four subtype C-specific nucleotides itis [Mochizuki et al., 1992], but the complete spectrum located within the positions 5708 to 6320 of the of diseases induced by HTLV-I is not fully defined. env gene. The nested PCR-RFLP method was used HTLV-I is endemic in southwestern Japan, the Caribto subtype HTLV-I from four virus-positive cell bean basin, tropical Africa, Central and South America, lines derived from 1 Japanese and 3 North Ameriand some regions of Melanesia [Hinuma et al., 1982; can patients, as well as 41 blood-unrelated Taiwan
De The ´et al., 1985;Manns and Blattner, 1991; Yanagi-Chinese. The sequences of PCR products were dehara et al., 1991].
According to phylogenetic analysis of the long termi-termined and the six positions of subtype-specific nal repeat (LTR) sequences of the virus, three types of nucleotide variations were examined. The se-HTLV-I have been proposed: 1) the Melanesian type quence data completely supported the subtyping from remote Melanesians in Papua New Guinea and data via the nested PCR-RFLP method. The results the Solomon Islands in the South Pacific Ocean and demonstrated that, as is the case in Japan, at least from Australian aboriginals [Gessain et al., 1991; Bastwo distinct cosmopolitan subtypes (A and B) of HTLV-I were present in Taiwan, but the more prevalent subtype in Taiwan is A in contrast to subtype Sponsored by National Science Council, 81-0412-B-002-48, 83-B in Japan. Furthermore, rapid subtyping could