Purification and characteristics of a sp
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Christina Psachoulia; Kostas Bourtzis; Dr. Vassilis J. Marmaras
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Article
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1989
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John Wiley and Sons
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English
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A specific alkaline phosphatase (ALPase) from t h e integument of white pupae has been purified 500-fold. The purification procedure included solubilization with Triton X-100, butanol extraction, fractionation with ammonium sulfate, and chromatography o n concanavalin A-Sepharose, Sephadex G-200, an