β Scribed by Yasuo Kagawa, Shigeo Ohta, Masafumi Yohda, Hajime Hirata, Toshiro Hamamoto (auth.), Sangkot Marzuki (eds.)
No coin nor oath required. For personal study only.
In recent years, the ATP synthase (H+Β·ATPase, FoFrATPase) has been the subject of intensive IDvestigations in many laboratories. The major reason for this lies in the fact that this enzyme complex catalyses one of the most important reactions in living cells, namely the synthesis of ATP utilizing the energy from an electrochemical transmembrane H+ gradient, generated by the cellular respiratory chain or by the light reactions of photosynthetic organisms. The mechanism by which the H+ motive force is utilized to drive the synthesis of ATP is one of the major unsolved problems in biochemistry. Thus, the fundamental information concerning the-molecular structure and the mechanism of assembly of the ATP synthase is of major significance in cell biology. A seminar/workshop on the Molecular Structure, Function and Assembly of the ATP synthases was held in April, 1987 at the EastΒ·West Center, University of Hawaii, Honolulu, Hawaii, to promote exchange of information between laboratories actively engaged in the study of the A TP synthases, and to provide a forum for discussion and coordination of data derived from molecular, genetic and biochemical approaches used in different laboratories. This volume summarizes the result of the seminar/workshop, in the form of a collection of papers presented at the meeting, and provides an overvIew of current work in this rapidly progressing area of research.
Front Matter....Pages i-ix
Front Matter....Pages 1-1
Gene Structure and Function of Thermophilic ATP Synthase....Pages 3-8
Genetic Studies of F 1 -ATPase of Escherichia Coli ....Pages 9-18
The Chloroplast Genes Encoding CF 0 Subunits of ATP Synthase....Pages 19-25
Expression and Evolution of the Chloroplast ATP Synthase Genes....Pages 27-33
Structure and Expression of Genes Encoding Higher Plant Mitochondrial F 1 F 0 -ATPase Subunits....Pages 35-42
Front Matter....Pages 43-43
The Assembly of the F 1 F 0 -ATPase Complex in Escherichia Coli ....Pages 45-49
Biochemical Analyses of oli1 and oli2 Gene Mutations Determining Primary Sequence Changes in Subunits 9 and 6 of Yeast ATP Synthase....Pages 51-65
The Structure and Expression of a Human Gene for a Nuclear-Coded Mitochondrial Adenosine Triphosphate Synthase Beta Subunit....Pages 67-72
Isolation of a cDNA Clone for the Ξ³ Subunit of the Chlamydomonas Reinhardtii CF 1 ....Pages 73-83
Import and Assembly of the Mitochondrial ATP Synthase of Bakerβs Yeast....Pages 85-93
Assembly of Yeast Mitochondrial ATP Synthase Incorporating an Imported Version of an F 0 Sector Subunit Normally Encoded within the Organelle....Pages 95-104
Front Matter....Pages 105-105
Structure of the Escherichia Coli ATP Synthase from Electron Microscopy Studies....Pages 107-114
Monoclonal Antibodies as Probes of Assembly of the Mitochondrial ATP Synthase....Pages 115-128
The Proton-ATPase of Chromaffin Granules and Synaptic Vesicles....Pages 129-139
A H + -Translocating ATP Synthase in an Extremely Halophilic Archaebacterium....Pages 141-147
Front Matter....Pages 149-149
Structure and Function of Mitochondrial Coupling Factor B(F B )....Pages 151-161
Subunit Arrangement in Bovine Mitochondrial H + -ATPASE....Pages 163-172
Interaction of Regulatory Subunits with the F 1 Sector of ATP Synthase in Mitochondria....Pages 173-180
The pKa Gate Mechanism for Protomotive Coupling: Effects of Internal Buffers on Initial Rates of ATP Synthesis....Pages 181-193
ATP Synthesis Driven by Intramembranal Protons....Pages 195-203
Front Matter....Pages 205-205
Catalysis by Isomeric Forms of Covalently Labeled F 1 -ATPase....Pages 207-217
Catalytic and Noncatalytic Nucleotide Binding Sites of F 1 -ATPases: Probes of Location, Structure and Function by Use of 2-N 3 -ATP....Pages 219-228
Interaction of Arylazido- Ξ² -Alanyl ATP a with the ATPase Enzyme of Rhodospirillum Rubrum Chromatophores....Pages 229-237
Catalytic Cooperativity in F 1 -ATPase: Photoaffinity Labeling Studies with BzATP a ....Pages 239-249
On the Number of Catalytic Sites in the F 1 -ATPase that Catalyze Steady State ATP Hydrolysis....Pages 251-258
The Effects of Neutral Salts and Nucleotides on the Stability of Beef Heart Mitochondrial F 1 -ATPase....Pages 259-266
Characterization of Nucleotide Binding Sites on Chloroplast Coupling Factor 1 (CF 1 ): Effects of Nucleotides on Nucleotide Triphosphate Formation by Isolated CF 1 ....Pages 267-271
A Hydrophobic Protein, Chargerin II, Purified from Rat Liver Mitochondria is Encoded in the Unidentified Reading Frame A6L of Mitochondrial DNA....Pages 273-280
Back Matter....Pages 281-284
Life Sciences, general
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