Molecular status of individual CFU-GM colonies derived from chemotherapy-mobilised peripheral blood stem cells in chronic myeloid leukaemia
✍ Scribed by Caroline M. Broughton; Paul Sherrington; Norman T. Pender; Richard E. Clark
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 135 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Following chemotherapy in chronic myeloid leukaemia (CML), some peripheral blood (PB) cells may be Philadelphia (Ph) chromosome negative. The BCR-ABL mRNA status of residual Ph 1 progenitors is not known. We examined the BCR-ABL mRNA status of individual colony-forming-unit granulocyte-macrophage (CFU-GM) colonies derived from PB harvested following chemotherapy. Seven patients were treated with 200 mg/m 2 /day cytarabine and 20 mg/m 2 /day Idarubicin and followed by Lenograstim. PB collections commenced daily when the white blood cell count reached 0.6 3 10 9 /l and continued until at least 5 3 10 8 /kg nucleated cells were obtained. CD34 1 cells, Ph status, and CFU-GM were estimated at each harvest. For each patient, up to 24 individual CFU-GM colonies were analysed for BCR-ABL status. Two cases were BCR-ABL negative on all colonies and completely Ph 2 , and another case was BCR-ABL positive in all colonies and completely Ph 1 . In contrast, in two patients all colonies were BCR-ABL negative, despite virtually complete Ph 1 metaphases. The final assessible case had five of nine