Molecular microbial diagnostic methods : pathways to implementation for the food and water industries

✍ Scribed by Cook, Nigel; D'Agostino, Martin; Thompson, Kenneth Clive

• Publisher: Academic Press is an imprint of Elsevier
• Year: 2015
• Tongue: English
• Leaves: 268
• Edition: 1
• Category: Library

✦ Synopsis

Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industry was developed by recognized and experienced highlevel scientists. It’s a comprehensive and detailed reference that uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. This book discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The content is also pertinent for anyone carrying out microbiological analysis at the research level, and for scientists developing methods, as it focuses on the requirements of end-users.

• Includes information on how to introduce and implement molecular methods for routine monitoring in food and water laboratories
• Discusses the importance of robust validation of molecular methods as alternatives to existing standard methods to help ensure the production of defendable results
• Highlights potential issues with respect to successful implementation of these methods

✦ Table of Contents

Content: Title page
Table of Contents
Copyright
Contributors
Preface
Chapter 1: Food industry current status
Abstract
Introduction
Molecular methods currently used
Laboratory automation in molecular methods
Challenges
Acceptance of use by the food industry
Compliance with current legal requirements
Disclaimer
Chapter 2: Future directions for molecular microbial diagnostic methods for the food industry
Abstract
Introduction
Evolution of food microbiology diagnostics: from petri dishes to PCR
Why introduce an alternative molecular diagnostic method? Characteristics of an ideal food molecular methodPast and current challenges
Current challenges
Concluding remarks
Chapter 3: Current status of molecular microbiological techniques for the analysis of drinking water
Abstract
Introduction and overview
The current state of play
The influence of standard and reference methods
Molecular techniques for testing potable water quality
Application of molecular techniques to wastewater
Conclusions
Chapter 4: What is now required for water?
Abstract
Introduction and overview
Indicator organisms
Pathogens in drinking water Total heterotrophic bacteriaFeasibility of the use of routine rapid water molecular methods
Future predictions
Conclusions
Chapter 5: CEN/ISO standards for both culture and molecular methods
Abstract
Introduction
Standards, standardization bodies, and structures in the microbiology of the food chain
Standards developed in the microbiology of the food chain
Status of novel technologies
Conclusions
Chapter 6: Laboratory validation, verification, and accreditation of molecular methods
Abstract
Alternative methods
Foundations of an accredited laboratory
Quality systems Cornerstones of accreditationMaintaining accreditation
Customer education
Health and safety
Chapter 7: DNA extraction: finding the most suitable method
Abstract
Boiling method
Column extraction
Magnetic beads
FTA™ cards
RNA extraction methods
Conclusions
Chapter 8: Assessing organism viability and interpreting genomic unit versus colony forming unit data for water and food borne microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria
Abstract
Introduction
Polymerase chain reaction
Assessing microbial viability
Overcoming the viability hurdle When to use viability discriminationStandardization and quality assurance
Proficiency testing
Conclusions
Chapter 9: MALDI-TOF MS: a rapid microbiological confirmation technique for food and water analysis
Abstract
Introduction and overview
Current confirmation methods and advantages of MALDI-TOF MS
MALDI-TOF MS Listeria speciation validation
Chapter 10: Chapter highlights, future requirements, and conclusions
Chapter highlights
Future requirements and conclusions
Index

✦ Subjects

Diagnostic microbiology -- Methodology. Molecular microbiology -- Methodology. SCIENCE / Life Sciences / Biology SCIENCE / Life Sciences / Microbiology