Molecular mechanisms of genetic recombination in bacteriophage: Joint molecules and their conversion to recombinant molecules
✍ Scribed by Jun-Ichi Tomizawa
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 1009 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Density-gradient centrifugation analysis of the DNA extracted from EscheTichia coli cells infected with a2P-and bromouracil-labeled phage and incubated with KCN showed the formation of linear molecules composed of two components derived from the parental DNA molecules joined end-to-end by hydrogen bonds (joint molecules). However, when the infected cells were incubated under conditions which permitted limited synthesis of phage DNA, the components which had been derived from the parental DNA were joined by covalent bonds to form recombinant molecules. It was also found that the structure of the majority of the chromosomes of mature phage T4, including heterozygous ones, could be represented by a duplex DNA molecule without an interruption in the strands. Studies on the genetic control of recombination of phage T4 showed that gene 32 has to be expressed to form joint molecules,