Molecular mapping of the chromosome 11 breakpoint of t(11;17)(q13;q21) in a t(11;14)(q13;q32)-positive B non-Hodgkin's lymphoma

✍ Scribed by Iwona Wlodarska; Eric Schoenmakers; Koen Kas; Jozef Merregaert; Vanessa Lemahieu; Ulli Weier; Herman Van Den Berghe; Dr. Wim J. M. Van De Ven

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 538 KB
• Volume: 8
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870080404

✦ Synopsis

The FAU gene is the cellular homologue of the viral FOX sequences in the genome of the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV); the viral FOX sequences have been shown to increase the transforming capacity of FBR-MuSV in vitro. The human FAU gene has recently been isolated, characterized, and mapped to chromosome band I I q I 3. Here, we report results of fluorescence in situ hybridization (FISH) analysis which indicate that the FAU gene maps proximally to the putative oncogene BCLl at I I ql3. Furthermore, we identified a t( I I ; I7)(q I 3;q2 I ) translocation in tumor cells of a t( I I ; I4)(q I 3;q32)-positive B-cell non-Hodgkin's lymphoma patient by FISH analysis using a FAU containing cosmid clone as molecular probe and by double-colour chromosome painting analysis using chromosome I Iand chromosome I7-specific painting probes. The position of the chromosome I I breakpoint of the t( I I ; 17) translocation was pinpointed to a human DNA region around the FAU gene of about 40 kbp.