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Molecular genotyping of HIV-1 in 61 patients with AIDS from Lom�, Togo

✍ Scribed by Davies, F.J.; d'Almeida, O.; Timmers, E.; d'Almeida, J.; Fasken, M.; Bassabi, K.; Lee, H.; Allain, J.-P.

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
229 KB
Volume
57
Category
Article
ISSN
0146-6615

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✦ Synopsis

To study the distribution of HIV types and genotypes, in Lome ´, Togo, a random population of patients who met the clinical criteria of the Bangui definition of AIDS and were positive with two independent screening assays for antibodies to HIV-1 group M, HIV-2, and HIV-1 group O was selected. HIV RNA from serum samples was reverse-transcribed and amplified with degenerate primers annealing to conserved regions of the HIV-1, HIV-2, and HIV-O gag gene. Amplicons were directly sequenced using an automated sequencer. A 262-271-bp (straindependent) fragment of the gag gene from each patient was phylogenetically analyzed and compared to the corresponding gag sequences of published HIV-1 sequences of known African genotypes. Genotype A was found in 48 of 60 patient amplicons (80%), subdivided into two clusters. Ten patients (16.7%) were HIV-1 genotype G; one was genotype D and one genotype H. HIV-1 genotype B was not found. Amplicons from two patients contained sequence ambiguities, requiring cloning and sequencing of the gag insert. One patient (T52) was apparently infected with HIV-1 genotypes A and G; whereas HIV-1 from patient T139 was of genotype A, with 2/10 clones having a three-codon insertion at nucleotide position 1142 of the gag gene. HIV-1 genotype A is dominant in Togo; genotype G is frequent and genotype B has not been found.

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