✦ LIBER ✦
Molecular genetics of rotifers: preliminary restriction napping of the mitochondrial genome ofBrachionus plicatilis
✍ Scribed by Charles E. King
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 476 KB
- Volume
- 186-187
- Category
- Article
- ISSN
- 1573-5141
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✦ Synopsis
Methods are presented to extract and purify mitochondrial DNA from the rotifer Brachionus plicatilis. The mtDNA obtained is of sufficient purity for digestion with restriction endonucleases. EcoR I restriction patterns are presented for 4 geographically separated clones. A restriction map based on digestion with 5 different restriction enzymes is included for one of these clones. Finally, use of mtDNA analysis for studies on the population structure and biogeography of rotifers is discussed.