A combination of Southern blotting and the analysis of polymerase chain reaction (PCR) amplified DNA fragments was used to screen the factor VIII genes of 527 haemophilia A patients for point mutations within TaqI restriction sites. Since this "directed search" strategy yielded only four gene lesion
β¦ LIBER β¦
Molecular genetics of coagulation factor VIII gene and haemophilia A
β Scribed by S. E. Antonarakis
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 493 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1351-8216
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## Abstract ## Background A gene therapy delivery system based on microcapsules enclosing recombinant cells engineered to secrete a therapeutic protein has been evaluated. The microcapsules are implanted intraperitoneally. In order to prevent cell immune rejection, cells are enclosed in nonβantige