Molecular forms of lipases and their localization in the fungusRhizopus microsporusby immuno-electron microscopy
✍ Scribed by J.K. Diyorov; K.A. Lusta; A.B. Tsiomenko; I.S. Kulaev
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 665 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1573-0972
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✦ Synopsis
Several lipases differing
in their molecular masses (24, 32, 43, 66 and 98 kDa and 28,40,45 and 69 kDa) were found in Rhizopus microsporus UzLT-4B and UzLT-5C, respectively. The lipases in each strain were immunologically related. Strain UzLT-5C grown on a medium with lipid substrate secreted lipases of 32,66 and 98 kDa whereas strain UzLT-4B produced lipases of 45 and 69 kDa on the same medium. Immuno-electron microscopy indicated that the intracellular lipases were in peripheral zones of the hyphae, primarily in the periplasm and adjacent vesicles. Immobilization in Ca-alginate gel revealed unusual structures in the cell wall. These structures accumulated lipases and, apparently, exported the enzymes out of the cell.