The application of atomic force microscopy (AFM) technique in proteomic research, identification and visualization of individual molecules and molecular complexes within the P450cam containing monooxygenase system was demonstrated. The method distinguishes between the binary protein complexes and ap
β¦ LIBER β¦
Molecular evolution of P450 superfamily and P450-containing monooxygenase systems
β Scribed by Kirill N. Degtyarenko; Alexander I. Archakov
- Book ID
- 115927640
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 973 KB
- Volume
- 332
- Category
- Article
- ISSN
- 0014-5793
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Trichosporon cutaneum metabolizes glucose purely oxidatively and cytochrome P450 was not detected in the reduced CO-difference spectrum of whole cells. However, in the isolated microsomal fraction the corresponding monooxygenase was present as shown by the appearance of cytochrome P450, NADPH-cytoch