High-frequency electron paramagnetic resonance (EPR) spectroscopy has been performed on a nitroxide spin-labeled peptide in fluid aqueous solution. The peptide, which follows the single letter sequence, was reacted with the methanethiosulfonate spin label at the cysteine sulfur. The spin sensitivity
Molecular dynamics simulation and EPR spectroscopy of nitroxide side chains in bacteriorhodopsin
✍ Scribed by Heinz-Jürgen Steinhoff; Matthias Müller; Christian Beier; Matthias Pfeiffer
- Book ID
- 104304070
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 668 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0167-7322
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EPR spectra of stable nitroxide radicals in fluid and in frozen micellar solutions of cationic and anionic surfactants were recorded. Analysis of the obtained spectra gave information on the mobility of molecules in the electrical double layer, in the core of the micelle and in the bulk water phase.
Gramicidin A (gA) is prototypical peptide antibiotic and a model ion channel former. Configured in the solid-state NMR beta(6.5)-helix channel conformation, gA was subjected to 1-ns molecular dynamics (MD) gas phase simulations using the all-atom charmm22 force field to ascertain the conformational