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Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis

✍ Scribed by Sriprapundh, D.; Vieille, C.; Zeikus, J.G.


Book ID
124079904
Publisher
Oxford University Press
Year
2000
Tongue
English
Weight
611 KB
Volume
13
Category
Article
ISSN
1741-0126

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## Abstract The folding of membrane proteins was addressed using outer membrane protein porin from the soil bacterium __Paracoccus denitrificans__ (__P. den.__). IR spectroscopy and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) analysis were used to probe the effect of mutage