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Molecular detection and sequencing of “Norwalk-like viruses” in outbreaks and sporadic cases of gastroenteritis in Ireland

✍ Scribed by Barbara Foley; Jim O'Mahony; Colin Hill; John G. Morgan

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
114 KB
Volume
65
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Norwalk‐like viruses (NLVs) are now established as the most important causative agents of epidemic gastroenteritis worldwide. The overall objective of this study was to determine the molecular epidemiology of Irish NLV isolates for the first time by obtaining sequence data from specimens originating from outbreaks and sporadic cases of gastroenteritis. Eight samples from sporadic cases of gastroenteritis and nine isolates from separate NLV outbreaks were examined. Of the sporadic isolates, six were shown to be genogroup 2 (G2) by RT‐PCR, while two were G1. All of the outbreak isolates were G2. All isolates were partially sequenced within a highly conserved region of ORF1 (RNA‐dependent RNA polymerase gene). Sequence data were aligned and a dendogram was constructed. The results indicated that the majority of G2 isolates were seen to cluster with Bristol and Lordsdale virus, while the two G1 specimens were related most closely to Southampton virus. Further downstream sequence analysis of a number of the isolates confirmed this result. It is concluded that the majority of NLV isolates circulating in Ireland belong to the Bristol/Lordsdale clade. J. Med. Virol. 65:388–394, 2001. © 2001 Wiley‐Liss, Inc.

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