A map showing locations of 22 of the 30 endonuclease EcoRI cleavage sites and 54 additional sites for eight other restriction endonucleases is presented. The regions of chloroplast DNA that hybridize with chloroplast tRNA are also shown.
Molecular cloning of the gene region coding for the chloroplast rRNA of Euglena gracilis
β Scribed by Knopf, Ulrich C. ;Stutz, Erhard
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 518 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
E. gracilis chloroplast DNA Bam fragments E and D, coding for rRNA were cloned separately using the plasmid pBR 322 as vector and E. coli as host. The newly constructed recombinant plasmids EgcKS 8 and EgcKS 11 (containing the Bam HI fragments E and D respectively) were analysed and characterized by gel electrophoresis, electronmicroscopy and analytical ultracentrifugation.
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