Molecular cloning of cDNAs encoding a putative cell wall protein fromZea maysand immunological identification of related polypeptides
✍ Scribed by Virginia Stiefel; Lluis Pérez-Grau; Fernando Albericio; Ernest Giralt; Luis Ruiz-Avila; M. Dolors Ludevid; Pere Puigdomènech
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 950 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
Copy DNAs corresponding to a highly repetitive, proline-rich protein from maize have been cloned by differential screening of a coleoptile cDNA library. The deduced amino acid sequence contains a single repetitive element of carrot extensin (Ser-Pro-Pro-Pro-Pro). The related mRNAs have a defined distribution in tissues of the plant and are accumulated mainly in the coleoptile node and root tip. A peptide that corresponds to one of the repetitive elements of the protein has been synthesized and antisera have been obtained in rabbits. These antibodies react against crude preparations of coleoptile cell wall and against polypeptides extracted following the protocols described for the extraction of extensin. From these data it is concluded that the cDNAs correspond to a family of cell wall glycoproteins from maize.