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Molecular cloning, gene structure, and expression pattern of pig immunoreceptor DAP12

โœ Scribed by Daesong Yim; Hyun-Bae Jie; Lewis L. Lanier; Y. B. Kim


Publisher
Springer-Verlag
Year
2000
Tongue
English
Weight
294 KB
Volume
51
Category
Article
ISSN
0093-7711

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The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di